2013
EMSS 2013 Proceeding
Modeling and simulation of amperometric biosensors acting in flow injection analysis
Authors: Romas Baronas, Darius Baronas
Abstract
This paper deals with amperometric biosensors acting in the flow injection mode when the biosensor contacts with an analyte for a short time. A biosensor-based an- alytical system is mathematically modeled by reaction- diffusion equations containing a non-linear term related to the Michaelis-Menten kinetics of an enzymatic reac- tion. The model involves four regions the enzyme layer where enzymatic reaction as well as the mass transport by diffusion take place, a dialysis membrane and a diffu- sion limiting region where only the diffusion take place, and a convective region where the analyte concentration is maintained constant. The system of equations was solved numerically by using the finite difference tech- nique. The biosensor operation is analyzed with a spe- cial emphasis to the effect of the dialysis membrane on the biosensor response. The biosensor sensitivity is in- vestigated by altering the model parameters influencing the thickness of the dialysis membrane and the catalytic activity of the enzyme. The half maximal effective con- centration of the analyte is used as a main characteristic of the sensitivity and the calibration curve of the biosen- sor.
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